Journal:

Article Title: Isolation and Characterization of Bacteria Capable of Tolerating the Extreme Conditions of Clean Room Environments ▿

doi: 10.1128/AEM.03007-06

Figure Lengend Snippet: Methodologies employed to cultivate several extremotolerant bacteria from spacecraft assembly facilities

Article Snippet: Samples were then subdivided for further processing, as shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Type of microbe Base medium a Alteration to medium and/or additional treatment Vol of sample (μl) Plating method Incubation temp (°C) Incubation time (days) Reference Heterotrophs R2A 100 Spread 25 7 12 Sporeformers TSA Heat shock b 5,000 Pour 32 7 18 Acidophiles R2A pH 3 c 100 Spread 25 7 30 Alkaliphiles R2A pH 9 d 100 Spread 25 7 30 Thermophiles R2A 100 Spread 65 4 This study Psychrophiles R2A 100 Spread 4 10 This study Halophiles R2A 25% NaCl e 100 Spread 25 7 This study Anaerobes Thio Incubated in anaerobic chamber f 100 Spread 25 7 This study UV resistant R2A 1,000 J/m 2 UV exposure g 5,000 Pour 25 7 21 H 2 O 2 resistant R2A One hour 5% H 2 O 2 incubation h 834 Pour 25 7 11 Open in a separate window a Three different base media were used: R2A, BD Difco R2A agar (catalog no. 218263); TSA, BD Difco tryptic soy agar (catalog no. 236950); Thio, BD BBL thioglycolate medium Brewer modified. b Five milliliters of sample was heat shocked for 15 minutes in an 80°C water bath prior to plating. c Four milliliters of a 0.5 M stock solution of citric acid to each 100 ml of R2A agar before pouring the plates.

Techniques: Incubation